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Immunoprecipitation Hepatocytes were lysed in an immunoprecipitation (IP) buffer (150 mM NaCl), 50 mM Tris-HCl, 1% Nonidet P-40, pH 7.8, and a mammalian cell-specific protease inhibitor cocktail

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[DOI] [PubMed] [Google Scholar] 157.Semchyshyn H

A patient injecting a compound of unknown purity at an unknown concentration is not running a self-directed protocol

Zheng G, Marino M, Zhao J, McCluskey RT

This design preserves long-term plasma exposure and steady-state signaling thresholds without requiring continuous infusion protocols